Identification of papillomaviruses in Antarctic pinnipeds sampled bet... - SRA

SRX20363457: Identification of papillomaviruses in Antarctic pinnipeds sampled between 2014 and 2018
1 ILLUMINA (Illumina HiSeq 4000) run: 14,448 spots, 2.9M bases, 1.3Mb downloads

Design: 200 l of UTM storage buffer in which the swabs were stored was used for viral DNA extraction using the High Pure Viral Nucleic Acid Kit (Roche Diagnostics, USA). 5l of the extracted viral DNA was amplified using the Illustra TempliPhi rolling circle amplification kit (GE Healthcare, USA) to target circular viruses. The viral DNA and rolling amplified viral DNA were combined and this was used to generate Illumina sequencing libraries using the TruSeq DNA Sample Prep Kit.

Submitted by: Arizona State University

Study: Towards understanding viral dynamics in Antarctic ecosystems

PRJNA874327 • SRP394564 • All experiments • All runs

show Abstracthide Abstract

Determination of viral genomes from various organisms and environmental sample in the Antarctic ecosystem

Sample:

SAMN35066574 • SRS17680369 • All experiments • All runs

Organism: Papillomavirus sp.

Library:

Name: LEO_100

Instrument: Illumina HiSeq 4000

Strategy: WGA